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小鼠中性粒細(xì)胞明膠酶相關(guān)脂質(zhì)運(yùn)載蛋白酶免試劑盒,(NGAL)ELISA檢測試劑盒 ,英文名:NGAL ELISA Kit ,英文縮寫:NGAL ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠中性粒細(xì)胞明膠酶相關(guān)脂質(zhì)運(yùn)載蛋白ELISA檢測試劑盒,NGAL 檢測試劑盒, NGAL 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠中性粒細(xì)胞明膠酶相關(guān)脂質(zhì)運(yùn)載蛋白酶免試劑盒,(NGAL)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
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成纖維細(xì)胞生長因子3(FGF3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 3 (FGF3)96TY17747Homo sapiens (Human)
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HtrA絲氨酸肽酶4(HTRA4)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for HtrA Serine Peptidase 4 (HTRA4)96TY17764Homo sapiens (Human)
紅細(xì)胞生成素(EPO)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Erythropoietin (EPO)96TY17765Rattus norvegicus (Rat)
胞外超氧化物歧化酶(SOD3)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Superoxide Dismutase 3, Extracellular (SOD3)96TY17766Mus musculus (Mouse)
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半乳糖凝集素9(GAL9)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Galectin 9 (GAL9)96TY17768Homo sapiens (Human)
補(bǔ)體成分5a(C5a)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Complement Component 5a (C5a)96TY17769Rattus norvegicus (Rat)
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Ⅰ型前膠原羧基端原肽(PⅠCP)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Procollagen I C-Terminal Propeptide (PICP)96TY17772Homo sapiens (Human)
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分泌型免疫球蛋白A(sIgA)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Secretory Immunoglobulin A (sIgA)96TY17774Bos taurus; Bovine (Cattle)
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C4結(jié)合蛋白β(C4BPβ)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for C4 Binding Protein Beta (C4BPb)96TY17777Homo sapiens (Human)
補(bǔ)體因子P(CFP)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Complement Factor P (CFP)96TY17778Homo sapiens (Human)
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各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。