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小鼠6酮前列腺素F1a酶免試劑盒,(6-keto-PGF1a)ELISA檢測試劑盒 ,英文名:6-keto-PGF1a ELISA Kit ,英文縮寫:6-keto-PGF1a ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠6酮前列腺素F1aELISA檢測試劑盒,6-keto-PGF1a 檢測試劑盒, 6-keto-PGF1a 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠6酮前列腺素F1a酶免試劑盒,(6-keto-PGF1a)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復(fù)性。
Aristaless樣同源框蛋白4(ALX4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Aristaless Like Homeobox Protein 4 (ALX4)96TY17546Mus musculus (Mouse)
Aristaless樣同源框蛋白4(ALX4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Aristaless Like Homeobox Protein 4 (ALX4)96TY17547Rattus norvegicus (Rat)
白介素1家族成員9(IL1F9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Family, Member 9 (IL1F9)96TY17548Homo sapiens (Human)
白介素1家族成員9(IL1F9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Family, Member 9 (IL1F9)96TY17549Mus musculus (Mouse)
白介素1家族成員9(IL1F9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 1 Family, Member 9 (IL1F9)96TY17550Rattus norvegicus (Rat)
泛素交叉反應(yīng)蛋白(UCRP)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)96TY17551Homo sapiens (Human)
泛素交叉反應(yīng)蛋白(UCRP)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)96TY17552Mus musculus (Mouse)
泛素交叉反應(yīng)蛋白(UCRP)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)96TY17553Rattus norvegicus (Rat)
干擾素α誘導(dǎo)蛋白27樣蛋白1(IFI27L1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Alpha Inducible Protein 27 Like Protein 1 (IFI27L1)96TY17554Homo sapiens (Human)
干擾素α誘導(dǎo)蛋白27樣蛋白2(IFI27L2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Alpha Inducible Protein 27 Like Protein 2 (IFI27L2)96TY17555Homo sapiens (Human)
干擾素α誘導(dǎo)蛋白27樣蛋白2(IFI27L2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Alpha Inducible Protein 27 Like Protein 2 (IFI27L2)96TY17556Mus musculus (Mouse)
干擾素α誘導(dǎo)蛋白27樣蛋白2(IFI27L2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interferon Alpha Inducible Protein 27 Like Protein 2 (IFI27L2)96TY17557Rattus norvegicus (Rat)
成纖維細胞生長因子4(FGF4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 4 (FGF4)96TY17558Bos taurus; Bovine (Cattle)
成纖維細胞生長因子4(FGF4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 4 (FGF4)96TY17559Chicken (Gallus)
成纖維細胞生長因子4(FGF4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 4 (FGF4)96TY17560Mus musculus (Mouse)
成纖維細胞生長因子4(FGF4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 4 (FGF4)96TY17561Rattus norvegicus (Rat)
成纖維細胞生長因子6(FGF6)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 6 (FGF6)96TY17562Danio rerio (Zebrafish)
成纖維細胞生長因子6(FGF6)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 6 (FGF6)96TY17563Homo sapiens (Human)
成纖維細胞生長因子6(FGF6)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 6 (FGF6)96TY17564Mus musculus (Mouse)
成纖維細胞生長因子6(FGF6)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 6 (FGF6)96TY17565Rattus norvegicus (Rat)
成纖維細胞生長因子9(FGF9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 9 (FGF9)96TY17566Bos taurus; Bovine (Cattle)
成纖維細胞生長因子9(FGF9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 9 (FGF9)96TY17567Chicken (Gallus)
成纖維細胞生長因子9(FGF9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 9 (FGF9)96TY17568Homo sapiens (Human)
成纖維細胞生長因子9(FGF9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 9 (FGF9)96TY17569Mus musculus (Mouse)
成纖維細胞生長因子9(FGF9)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 9 (FGF9)96TY17570Rattus norvegicus (Rat)
FMS樣酪氨酸激酶3配體(Flt3L)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for FMS Like Tyrosine Kinase 3 Ligand (Flt3L)96TY17571Homo sapiens (Human)
FMS樣酪氨酸激酶3配體(Flt3L)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for FMS Like Tyrosine Kinase 3 Ligand (Flt3L)96TY17572Mus musculus (Mouse)
中性粒細胞激活蛋白3(NAP3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Neutrophil Activating Protein 3 (NAP3)96TY17573Homo sapiens (Human)
中性粒細胞激活蛋白3(NAP3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Neutrophil Activating Protein 3 (NAP3)96TY17574Mus musculus (Mouse)
中性粒細胞激活蛋白3(NAP3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Neutrophil Activating Protein 3 (NAP3)96TY17575Rattus norvegicus (Rat)
白介素3調(diào)節(jié)核因子(NFIL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Nuclear Factor, Interleukin 3 Regulated (NFIL3)96TY17576Homo sapiens (Human)
白介素3調(diào)節(jié)核因子(NFIL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Nuclear Factor, Interleukin 3 Regulated (NFIL3)96TY17577Mus musculus (Mouse)
白介素3調(diào)節(jié)核因子(NFIL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Nuclear Factor, Interleukin 3 Regulated (NFIL3)96TY17578Rattus norvegicus (Rat)
PDGFA關(guān)聯(lián)蛋白1(PDAP1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for PDGFA Associated Protein 1 (PDAP1)96TY17579Homo sapiens (Human)
PDGFA關(guān)聯(lián)蛋白1(PDAP1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for PDGFA Associated Protein 1 (PDAP1)96TY17580Mus musculus (Mouse)
PDGFA關(guān)聯(lián)蛋白1(PDAP1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for PDGFA Associated Protein 1 (PDAP1)96TY17581Rattus norvegicus (Rat)
小斑型POZ蛋白(SPOP)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Speckle Type POZ Protein (SPOP)96TY17582Homo sapiens (Human)
小斑型POZ蛋白(SPOP)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Speckle Type POZ Protein (SPOP)96TY17583Mus musculus (Mouse)
小斑型POZ蛋白(SPOP)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Speckle Type POZ Protein (SPOP)96TY17584Rattus norvegicus (Rat)
正輔因子4(PC4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Positive Cofactor 4 (PC4)96TY17585Homo sapiens (Human)
正輔因子4(PC4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Positive Cofactor 4 (PC4)96TY17586Mus musculus (Mouse)
正輔因子4(PC4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Positive Cofactor 4 (PC4)96TY17587Rattus norvegicus (Rat)
胰島素生長因子樣家族成員2(IGFL2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 2 (IGFL2)96TY17588Homo sapiens (Human)
胰島素生長因子樣家族成員3(IGFL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 3 (IGFL3)96TY17589Homo sapiens (Human)
胰島素生長因子樣家族成員3(IGFL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 3 (IGFL3)96TY17590Mus musculus (Mouse)
胰島素生長因子樣家族成員3(IGFL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 3 (IGFL3)96TY17591Rattus norvegicus (Rat)
胰島素生長因子樣家族成員4(IGFL4)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 4 (IGFL4)96TY17592Homo sapiens (Human)
胰島素生長因子樣家族成員1(IGFL1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for IGF Like Family, Member 1 (IGFL1)96TY17593Homo sapiens (Human)
趨化素樣因子超家族成員8(CKLFSF8)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Chemokine Like Factor Superfamily 8 (CKLFSF8)96TY17594Homo sapiens (Human)
趨化素樣因子超家族成員8(CKLFSF8)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Chemokine Like Factor Superfamily 8 (CKLFSF8)96TY17595Mus musculus (Mouse)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。