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小鼠轉(zhuǎn)化生長因子β2酶免試劑盒,(TGFβ2)ELISA檢測試劑盒 ,英文名:TGFβ2 ELISA Kit ,英文縮寫:TGFβ2 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠轉(zhuǎn)化生長因子β2ELISA檢測試劑盒,TGFβ2 檢測試劑盒, TGFβ2 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠轉(zhuǎn)化生長因子β2酶免試劑盒,(TGFβ2)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
補體1q(C1q)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement 1q (C1q)48T/96TY16685Mus musculus (Mouse)
N-甲基-D-天氡氨酸離子能谷氨酸受體2C(GRIN2C)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C)48T/96TY16686Homo sapiens (Human)
N-甲基-D-天氡氨酸離子能谷氨酸受體2B(GRIN2B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B)48T/96TY16687Homo sapiens (Human)
鞘氨醇-1-磷酸磷酸酶2(SGPP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sphingosine-1-Phosphate Phosphatase 2 (SGPP2)48T/96TY16688Rattus norvegicus (Rat)
谷氨酸半胱氨酸連接酶(GCLM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Cysteine Ligase, Modifier Subunit (GCLM)48T/96TY16689Rattus norvegicus (Rat)
鞘氨醇-1-磷酸磷酸酶1(SGPP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sphingosine-1-Phosphate Phosphatase 1 (SGPP1)48T/96TY16690Rattus norvegicus (Rat)
骨涎蛋白(BSP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Sialoprotein (BSP)48T/96TY16691Mus musculus (Mouse)
鈣調(diào)結(jié)合蛋白(CALD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caldesmon (CALD)48T/96TY16692Rattus norvegicus (Rat)
套膜蛋白(iNV)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Involucrin (iNV)48T/96TY16693Mus musculus (Mouse)
脊髓灰質(zhì)炎病毒受體相關(guān)分子1(PVRL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Poliovirus Receptor Related Protein 1 (PVRL1)48T/96TY16694Homo sapiens (Human)
S100鈣結(jié)合蛋白A4(S100A4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A4 (S100A4)48T/96TY16695Mus musculus (Mouse)
泛素結(jié)合酶E2C結(jié)合蛋白E2L3(UBE2L3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin Conjugating Enzyme E2L3 (UBE2L3)48T/96TY16696Homo sapiens (Human)
法尼酯X受體(FXR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Farnesoid X Receptor (FXR)48T/96TY16697Rattus norvegicus (Rat)
鞘氨醇-1-磷酸磷酸酶1(SGPP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sphingosine-1-Phosphate Phosphatase 1 (SGPP1)48T/96TY16698Homo sapiens (Human)
Ⅵ型膠原α1(COL6α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type VI Alpha 1 (COL6a1)48T/96TY16699Rattus norvegicus (Rat)
神經(jīng)調(diào)節(jié)素2(NRG2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuregulin 2 (NRG2)48T/96TY16700Rattus norvegicus (Rat)
小異二聚體伴侶(SHP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Small Heterodimer Partner (SHP)48T/96TY16701Homo sapiens (Human)
吲哚胺2,3-雙加氧酶2(IDO2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Indoleamine-2,3-Dioxygenase 2 (IDO2)48T/96TY16702Mus musculus (Mouse)
丁酰膽堿酯酶(BCHE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Butyrylcholinesterase (BCHE)48T/96TY16703Rattus norvegicus (Rat)
甲基CpG結(jié)合蛋白2(MECP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Methyl CpG Binding Protein 2 (MECP2)48T/96TY16704Homo sapiens (Human)
興奮性氨基酸轉(zhuǎn)運蛋白3(EAAT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Excitatory Amino Acid Transporter 3 (EAAT3)48T/96TY16705Homo sapiens (Human)
蘭尼定受體1(RYR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ryanodine Receptor 1, Skeletal (RYR1)48T/96TY16706Homo sapiens (Human)
Smoothelin蛋白(SMTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Smoothelin (SMTN)48T/96TY16707Rattus norvegicus (Rat)
死亡誘導(dǎo)終結(jié)因子1(DIDO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Death Inducer Obliterator 1 (DIDO1)48T/96TY16708Homo sapiens (Human)
死亡誘導(dǎo)終結(jié)因子1(DIDO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Death Inducer Obliterator 1 (DIDO1)48T/96TY16709Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子17(FGF17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 17 (FGF17)48T/96TY16710Homo sapiens (Human)
鈣蛋白酶10(CAPN10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calpain 10 (CAPN10)48T/96TY16711Mus musculus (Mouse)
Apelin蛋白(APLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apelin (APLN)48T/96TY16712Rattus norvegicus (Rat)
VI型膠原(COL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type VI (COL6)48T/96TY16713Mus musculus (Mouse)
肌球蛋白重鏈11(MYH11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 11, Smooth Muscle (MYH11)48T/96TY16714Homo sapiens (Human)
蛋白酪氨酸磷酸酶樣蛋白A(PTPLA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase Like Protein A (PTPLA)48T/96TY16715Homo sapiens (Human)
非受體型蛋白酪氨酸磷酸酶6(PTPN6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6)48T/96TY16716Homo sapiens (Human)
細(xì)胞色素P450家族成員27B1(CYP27B1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome P450 27B1 (CYP27B1)48T/96TY16717Mus musculus (Mouse)
維甲酸受體β(RARβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Retinoic Acid Receptor Beta (RARb)48T/96TY16718Homo sapiens (Human)
維甲酸受體γ(RARγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Retinoic Acid Receptor Gamma (RARg)48T/96TY16719Homo sapiens (Human)
肝受體同源物1(LRH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Liver Receptor Homolog 1 (LRH1)48T/96TY16720Homo sapiens (Human)
肝受體同源物1(LRH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Liver Receptor Homolog 1 (LRH1)48T/96TY16721Rattus norvegicus (Rat)
囊泡單胺轉(zhuǎn)運蛋白2(VMAT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vesicular Monoamine Transporter 2 (VMAT2)48T/96TY16722Homo sapiens (Human)
陽離子氨基酸轉(zhuǎn)運蛋白2(CAT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cationic Amino Acid Transporter 2 (CAT2)48T/96TY16723Rattus norvegicus (Rat)
N-?;掖及匪狨;福∟AAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-Acylethanolamine Acid Amidase (NAAA)48T/96TY16724Homo sapiens (Human)
真核翻譯起始因子4A2(EIF4A2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Eukaryotic Translation Initiation Factor 4A2 (EIF4A2)48T/96TY16725Homo sapiens (Human)
DNA指導(dǎo)聚合酶γ1(POLγ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Polymerase DNA Directed Gamma 1 (POLg1)48T/96TY16726Homo sapiens (Human)
腺苷A2b受體(ADORA2b)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenosine A2b Receptor (ADORA2b)48T/96TY16727Mus musculus (Mouse)
磷脂酰肌醇結(jié)合網(wǎng)格蛋白裝配蛋白(PICALM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM)48T/96TY16728Homo sapiens (Human)
氧化應(yīng)激誘導(dǎo)生長抑制因子1(OSGIN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1)48T/96TY16729Homo sapiens (Human)
B-Raf原癌基因絲氨酸/蘇氨酸蛋白激酶(BRAF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)48T/96TY16730Homo sapiens (Human)
內(nèi)質(zhì)網(wǎng)氨肽酶2(ERAP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endoplasmic Reticulum Aminopeptidase 2 (ERAP2)48T/96TY16731Homo sapiens (Human)
甘氨酸脫氫酶(GLDC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycine Dehydrogenase (GLDC)48T/96TY16732Mus musculus (Mouse)
甲狀腺轉(zhuǎn)錄因子1(TITF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)小鼠轉(zhuǎn)化生長因子β2酶免試劑盒,(TGFβ2)ELISA檢測試劑盒ELISA Kit for Thyroid Transcription Factor 1 (TITF1)48T/96TY16733Homo sapiens (Human)
腫瘤蛋白p53結(jié)合蛋白1(TP53BP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Protein p53 Binding Protein 1 (TP53BP1)48T/96TY16734Homo sapiens (Human)
各種類型ELISA測定時,小鼠轉(zhuǎn)化生長因子β2酶免試劑盒,(TGFβ2)ELISA檢測試劑盒一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。