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小鼠鉤端螺旋體IgG酶免試劑盒,(Lep IgG)ELISA檢測(cè)試劑盒 ,英文名:Lep IgG ELISA Kit ,英文縮寫:Lep IgG ,常見試劑盒規(guī)格:96T,產(chǎn)品別名:小鼠鉤端螺旋體IgGELISA檢測(cè)試劑盒,Lep IgG 檢測(cè)試劑盒, Lep IgG 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠鉤端螺旋體IgG酶免試劑盒,(Lep IgG)ELISA檢測(cè)試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過(guò)程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無(wú)關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過(guò)程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
溶質(zhì)載體家族30成員4(SLC30A4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Solute Carrier Family 30 Member 4 (SLC30A4)48T/96TY16485Mus musculus (Mouse)
溶質(zhì)載體家族30成員7(SLC30A7)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Solute Carrier Family 30 Member 7 (SLC30A7)48T/96TY16486Mus musculus (Mouse)
鞘氨醇1磷酸酯裂解酶1(SGPL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Sphingosine 1 Phosphate Lyase 1 (SGPL1)48T/96TY16487Mus musculus (Mouse)
11-β-羥基類固醇脫氫酶2(HSD11β2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2)48T/96TY16488Homo sapiens (Human)
L-型電壓依賴鈣離子通道α1S亞基(CACNα1S)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Calcium Channel, Voltage Dependent, L-Type, Alpha 1S Subunit (CACNa1S)48T/96TY16489Mus musculus (Mouse)
L-型電壓依賴鈣離子通道α1C亞基(CACNα1C)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C)48T/96TY16490Mus musculus (Mouse)
L-型電壓依賴性鈣離子通道α1D亞基(CACNα1D)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D)48T/96TY16491Mus musculus (Mouse)
L-型電壓依賴鈣離子通道α1F亞基(CACNα1F)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Calcium Channel, Voltage Dependent, L-Type, Alpha 1F Subunit (CACNa1F)48T/96TY16492Mus musculus (Mouse)
N-型電壓依賴鈣離子通道α1B亞基(CACNα1B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B)48T/96TY16493Mus musculus (Mouse)
R-型電壓依賴鈣離子通道α1E亞基(CACNα1E)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E)48T/96TY16494Mus musculus (Mouse)
T-型電壓依賴鈣離子通道α1H亞基(CACNα1H)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Calcium Channel, Voltage Dependent, T-Type, Alpha 1H Subunit (CACNa1H)48T/96TY16495Mus musculus (Mouse)
端粒重復(fù)結(jié)合因子2(TERF2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for omeric Repeat Binding Factor 2 (TERF2)48T/96TY16496Homo sapiens (Human)
過(guò)氧化還原酶6(PRDX6)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Peroxiredoxin 6 (PRDX6)48T/96TY16497Mus musculus (Mouse)
鈉/鉀離子轉(zhuǎn)運(yùn)ATP酶α1肽(ATP1α1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)48T/96TY16498Rattus norvegicus (Rat)
鈉/鉀離子轉(zhuǎn)運(yùn)ATP酶β1肽(ATP1β1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1)48T/96TY16499Rattus norvegicus (Rat)
溶質(zhì)載體家族30成員3(SLC30A3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Solute Carrier Family 30, Member 3 (SLC30A3)48T/96TY16500Mus musculus (Mouse)
溶質(zhì)載體家族30成員5(SLC30A5)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Solute Carrier Family 30, Member 5 (SLC30A5)48T/96TY16501Mus musculus (Mouse)
溶質(zhì)載體家族30成員6(SLC30A6)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Solute Carrier Family 30, Member 6 (SLC30A6)48T/96TY16502Mus musculus (Mouse)
肝細(xì)胞核因子1β(HNF1β)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Hepatocyte Nuclear Factor 1 Beta (HNF1b)48T/96TY16503Rattus norvegicus (Rat)
丙糖磷酸異構(gòu)酶1(TPI1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Triosephosphate Isomerase 1 (TPI1)48T/96TY16504Mus musculus (Mouse)
墨蝶呤還原酶(SPR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Sepiapterin Reductase (SPR)48T/96TY16505Mus musculus (Mouse)
亮氨酸豐富重復(fù)FLII相互作用蛋白1(LRRFIP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1)48T/96TY16506Homo sapiens (Human)
端粒保護(hù)1同源物(POT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protection Of omeres 1 Homolog (POT1)48T/96TY16507Homo sapiens (Human)
含亮氨酸豐富重復(fù)蛋白3C(LRRC3C)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Leucine Rich Repeat Containing Protein 3C (LRRC3C)48T/96TY16508Homo sapiens (Human)
FK506結(jié)合蛋白樣蛋白(FKBPL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for FK506 Binding Protein Like Protein (FKBPL)48T/96TY16509Mus musculus (Mouse)
無(wú)翅型MMTV整合位點(diǎn)家族成員4(WNT4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 4 (WNT4)48T/96TY16510Homo sapiens (Human)
含RCC1和BTB域蛋白2(RCBTB2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for RCC1 And BTB Domain Containing Protein 2 (RCBTB2)48T/96TY16511Homo sapiens (Human)
類固醇5α還原酶2(SRD5α2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2)48T/96TY16512Homo sapiens (Human)
含SET域蛋白7(SETD7)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for SET Domain Containing Protein 7 (SETD7)48T/96TY16513Homo sapiens (Human)
赫曼斯基普德拉克綜合征蛋白6(HPS6)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Hermansky Pudlak Syndrome Protein 6 (HPS6)48T/96TY16514Homo sapiens (Human)
絲氨酸蛋白酶50(PRSS50)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protease, Serine 50 (PRSS50)48T/96TY16515Homo sapiens (Human)
含Toll白細(xì)胞介素1受體域銜接蛋白(TIRAP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP)48T/96TY16516Homo sapiens (Human)
肌球蛋白重鏈7B(MYH7B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B)48T/96TY16517Homo sapiens (Human)
無(wú)翅型MMTV整合位點(diǎn)家族成員9B(WNT9B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 9B (WNT9B)48T/96TY16518Homo sapiens (Human)
T-框蛋白21(TBX21)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for T-Box Protein 21 (TBX21)48T/96TY16519Homo sapiens (Human)
粘蛋白12(MUC12)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Mucin 12, Cell Surface Associated (MUC12)48T/96TY16520Homo sapiens (Human)
鈉/鉀離子轉(zhuǎn)運(yùn)ATP酶β4肽(ATP1β4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4)48T/96TY16521Rattus norvegicus (Rat)
棘皮動(dòng)物微管關(guān)聯(lián)蛋白樣蛋白2(EML2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Echinoderm Microtubule Associated Protein Like Protein 2 (EML2)48T/96TY16522Homo sapiens (Human)
胎盤特異性蛋白9(PLAC9)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Placenta Specific Protein 9 (PLAC9)48T/96TY16523Homo sapiens (Human)
短腭肺鼻腔上皮癌關(guān)聯(lián)蛋白2(SPLUNC2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2)48T/96TY16524Homo sapiens (Human)
短腭肺鼻腔上皮癌關(guān)聯(lián)蛋白3(SPLUNC3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 (SPLUNC3)48T/96TY16525Homo sapiens (Human)
抑制素βC(INHβC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Inhibin Beta C (INHbC)48T/96TY16526Mus musculus (Mouse)
銅伴侶超氧化物歧化酶(SOD4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Superoxide Dismutase, Copper Chaperone (SOD4)48T/96TY16527Homo sapiens (Human)
胱天蛋白酶3(CASP3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caspase 3 (CASP3)48T/96TY16528Bos taurus; Bovine (Cattle)
補(bǔ)體受體2(CR2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Complement Receptor 2 (CR2)48T/96TY16529Rattus norvegicus (Rat)
細(xì)胞程序性死亡蛋白1(PDCD1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Programmed Cell Death Protein 1 (PDCD1)48T/96TY16530Homo sapiens (Human)
肌球蛋白重鏈2(MYH2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)48T/96TY16531Homo sapiens (Human)
B-細(xì)胞淋巴瘤因子2(Bcl2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2)48T/96TY16532Bos taurus; Bovine (Cattle)
甲狀旁腺激素相關(guān)蛋白(PTHrP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Parathyroid Hormone Related Protein (PTHrP)48T/96TY16533Bos taurus; Bovine (Cattle)
整合素α2(ITGα2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Integrin Alpha 2 (ITGa2)48T/96TY16534Rattus norvegicus (Rat)
各種類型ELISA測(cè)定時(shí), 一般需經(jīng)過(guò)以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。