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小鼠血管緊張素轉(zhuǎn)化酶酶免試劑盒,(ACE)ELISA檢測試劑盒,英文名:ACE ELISA Kit,英文縮寫:ACE ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠血管緊張素轉(zhuǎn)化酶ELISA檢測試劑盒,ACE 檢測試劑盒,ACE 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠血管緊張素轉(zhuǎn)化酶酶免試劑盒,(ACE)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
鈷胺傳遞蛋白Ⅱ(TCN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transcobalamin II, Macrocytic Anemia (TCN2)48T/96TY13534Homo sapiens (Human)
血小板反應(yīng)蛋白4(THBS4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thrombospondin 4 (THBS4)48T/96TY13535Mus musculus (Mouse)
血小板反應(yīng)蛋白3(THBS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thrombospondin 3 (THBS3)48T/96TY13536Mus musculus (Mouse)
外周鞘磷脂蛋白22(PMP22)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peripheral Myelin Protein 22 (PMP22)48T/96TY13537Mus musculus (Mouse)
外周鞘磷脂蛋白22(PMP22)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peripheral Myelin Protein 22 (PMP22)48T/96TY13538Rattus norvegicus (Rat)
1號染色體開放閱讀框85(C1orf85)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chromosome 1 Open Reading Frame 85 (C1orf85)48T/96TY13539Mus musculus (Mouse)
醌NADH脫氫酶1(NQO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for NADH Dehydrogenase, Quinone 1 (NQO1)48T/96TY13540Rattus norvegicus (Rat)
葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose Transporter 4 (GLUT4)48T/96TY13541Canis familiaris; Canine (Dog)
脂素1(LPIN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipin 1 (LPIN1)48T/96TY13542Mus musculus (Mouse)
卵泡抑素樣蛋白3(FSTL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Follistatin Like Protein 3 (FSTL3)48T/96TY13543Mus musculus (Mouse)
甲硫氨酰氨肽酶2(METAP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Methionyl Aminopeptidase 2 (METAP2)48T/96TY13544Homo sapiens (Human)
神經(jīng)肽Y受體Y2(NPY2R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuropeptide Y Receptor Y2 (NPY2R)48T/96TY13545Mus musculus (Mouse)
神經(jīng)肽Y受體Y1(NPY1R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuropeptide Y Receptor Y1 (NPY1R)48T/96TY13546Mus musculus (Mouse)
Anoctamin 6蛋白(ANO6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anoctamin 6 (ANO6)48T/96TY13547Homo sapiens (Human)
白三烯A4水解酶(LTA4H)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukotriene A4 Hydrolase (LTA4H)48T/96TY13548Mus musculus (Mouse)
凝血因子Ⅺ(F11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor XI (F11)48T/96TY13549Rattus norvegicus (Rat)
肽聚糖識別蛋白1(PGLYRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peptidoglycan Recognition protein 1 (PGLYRP1)48T/96TY13550Mus musculus (Mouse)
17-β-羥基類固醇脫氫酶3(HSD17β3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)48T/96TY13551Homo sapiens (Human)
C-Raf原癌基因絲蘇氨酸蛋白激酶(CRAF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF)48T/96TY13552Homo sapiens (Human)
野鼠色基因相關(guān)蛋白(AGRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Agouti Related Protein (AGRP)48T/96TY13553Mus musculus (Mouse)
前膠原C端蛋白酶增強(qiáng)子1(PCPE1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen C Proteinase Enhancer 1 (PCPE1)48T/96TY13554Mus musculus (Mouse)
腫瘤壞死因子配體超家族成員13(TNFSF13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)48T/96TY13555Mus musculus (Mouse)
血小板反應(yīng)蛋白4(THBS4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thrombospondin 4 (THBS4)48T/96TY13556Homo sapiens (Human)
轉(zhuǎn)化受體電位陽離子通道亞家族V成員1(TRPV1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1)48T/96TY13557Homo sapiens (Human)
早幼粒細(xì)胞白血病蛋白(PML)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Promyelocytic Leukemia Protein (PML)48T/96TY13558Homo sapiens (Human)
細(xì)胞胱硫醚γ裂解酶(CSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cystathionine Gamma Lyase (CSE)48T/96TY13559Rattus norvegicus (Rat)
腱蛋白樣蛋白1(CHRDL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chordin Like Protein 1 (CHRDL1)48T/96TY13560Homo sapiens (Human)
成纖維細(xì)胞激活蛋白α(FAPα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Activation Protein Alpha (FAPa)48T/96TY13561Mus musculus (Mouse)
NFKB抑制因子(NKRF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for NFKB Repressing Factor (NKRF)48T/96TY13562Homo sapiens (Human)
饑餓素-O-乙酰基轉(zhuǎn)移酶(GOAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ghrelin-O-Acyltransferase (GOAT)48T/96TY13563Homo sapiens (Human)
胸腺基質(zhì)淋巴細(xì)胞生成素(TSLP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thymic Stromal Lymphopoietin (TSLP)48T/96TY13564Rattus norvegicus (Rat)
卵磷脂膽固醇脂酰轉(zhuǎn)移酶(LCAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lecithin Cholesterol Acyltransferase (LCAT)48T/96TY13565Mus musculus (Mouse)
CREB結(jié)合蛋白(CREBBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for CREB Binding Protein (CREBBP)48T/96TY13566Homo sapiens (Human)
膜鐵轉(zhuǎn)運(yùn)蛋白(FPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ferroportin (FPN)48T/96TY13567Homo sapiens (Human)
Dickkopf相關(guān)蛋白1(DKK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dickkopf Related Protein 1 (DKK1)48T/96TY13568Rattus norvegicus (Rat)
白介素6受體(IL6R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 6 Receptor (IL6R)48T/96TY13569Mus musculus (Mouse)
分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secreted Frizzled Related Protein 1 (SFRP1)48T/96TY13570Rattus norvegicus (Rat)
嗜酸性粒細(xì)胞過氧化物酶(EPX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Eosinophil Peroxidase (EPX)48T/96TY13571Mus musculus (Mouse)
雙腎上腺皮質(zhì)激素(DCX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Doublecortin (DCX)48T/96TY13572Rattus norvegicus (Rat)
肝配蛋白B受體3(EPHB3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ephrin Type B Receptor 3 (EPHB3)48T/96TY13573Rattus norvegicus (Rat)
肌微管素相關(guān)蛋白9(MTMR9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myotubularin Related Protein 9 (MTMR9)48T/96TY13574Rattus norvegicus (Rat)
轉(zhuǎn)化受體電位陽離子通道亞家族M成員4(TRPM4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4)48T/96TY13575Rattus norvegicus (Rat)
復(fù)制啟動因子1(REPIN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Replication Initiator 1 (REPIN1)48T/96TY13576Homo sapiens (Human)
復(fù)制啟動因子1(REPIN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Replication Initiator 1 (REPIN1)48T/96TY13577Mus musculus (Mouse)
核因子相關(guān)κB結(jié)合蛋白(NFRkB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor Related To Kappa B Binding Protein (NFRkB)48T/96TY13578Homo sapiens (Human)
Discs大同源物關(guān)聯(lián)蛋白5(DLGAP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Discs, Large Homolog Associated Protein 5 (DLGAP5)48T/96TY13579Homo sapiens (Human)
轉(zhuǎn)錄激活因子3(ATF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activating Transcription Factor 3 (ATF3)48T/96TY13580Mus musculus (Mouse)
無翅型MMTV整合位點家族成員5B(WNT5B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 5B (WNT5B)48T/96TY13581Homo sapiens (Human)
含動力蛋白重鏈域蛋白1(DNHD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dynein Heavy Chain Domain Containing Protein 1 (DNHD1)48T/96TY13582Homo sapiens (Human)
唾液酸結(jié)合Ig樣凝集素8(SIGLEC8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8)48T/96TY13583Homo sapiens (Human)
異粘蛋白(MTDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Metadherin (MTDH)48T/96TY13584Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。