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小鼠c-jun ELISA檢測試劑盒 ,英文名:小鼠c-jun ELISA試劑盒 ,英文縮寫:小鼠c-jun ELISA試劑盒 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠c-jun ELISA試劑盒 小鼠c-junELISA試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠c-jun ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復(fù)性。
再生胰島衍生蛋白1β(REG1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 1 Beta (REG1b)48T/96TY13035Homo sapiens (Human)
賴氨酰氧化酶樣蛋白2(LOXL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysyl Oxidase Like Protein 2 (LOXL2)48T/96TY13036Homo sapiens (Human)
鈣同線蛋白2(CLSTN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calsyntenin 2 (CLSTN2)48T/96TY13037Homo sapiens (Human)
甘露糖結(jié)合凝集素2(LMAN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lectin, Mannose Binding 2 (LMAN2)48T/96TY13038Homo sapiens (Human)
鈣調(diào)寧蛋白1(CNN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calponin 1, Basic (CNN1)48T/96TY13039Homo sapiens (Human)
蛋白Z(PROZ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Z (PROZ)48T/96TY13040Mus musculus (Mouse)
促腎上腺皮質(zhì)素釋放激素結(jié)合蛋白(CRHBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Corticotropin Releasing Hormone Binding Protein (CRHBP)48T/96TY13041Homo sapiens (Human)
WNT1誘導(dǎo)信號通道蛋白1(WISP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for WNT1 Inducible Signaling Pathway Protein 1 (WISP1)48T/96TY13042Mus musculus (Mouse)
內(nèi)皮素2(EDN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endothelin 2 (EDN2)48T/96TY13043Rattus norvegicus (Rat)
血管舒張劑激活磷蛋白(VASP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vasodilator Stimulated Phosphoprotein (VASP)48T/96TY13044Homo sapiens (Human)
神經(jīng)疾病靶標酯酶(NTE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuropathy Target Esterase (NTE)48T/96TY13045Rattus norvegicus (Rat)
整合素αV(ITGαV)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Integrin Alpha V (ITGaV)48T/96TY13046Homo sapiens (Human)
基質(zhì)重塑關(guān)聯(lián)蛋白5(MXRA5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Remodelling Associated Protein 5 (MXRA5)48T/96TY13047Homo sapiens (Human)
斯鈣素1(STC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Stanniocalcin 1 (STC1)48T/96TY13048Mus musculus (Mouse)
Klotho蛋白(KL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Klotho (KL)48T/96TY13049Rattus norvegicus (Rat)
層黏連蛋白受體1(LAMR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Laminin Receptor 1 (LAMR1)48T/96TY13050Homo sapiens (Human)
心營養(yǎng)素樣細胞因子1(CLCF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cardiotrophin Like Cytokine Factor 1 (CLCF1)48T/96TY13051Homo sapiens (Human)
細胞骨架活性調(diào)節(jié)蛋白(ARC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activity Regulated Cytoskeleton Associated Protein (ARC)48T/96TY13052Mus musculus (Mouse)
生長分化因子3(GDF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 3 (GDF3)48T/96TY13053Mus musculus (Mouse)
生長分化因子3(GDF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 3 (GDF3)48T/96TY13054Rattus norvegicus (Rat)
多巴胺轉(zhuǎn)運蛋白(DAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Transporter (DAT)48T/96TY13055Homo sapiens (Human)
異檸檬酸脫氫酶1(IDH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Isocitrate Dehydrogenase 1, Soluble (IDH1)48T/96TY13056Homo sapiens (Human)
骨髓基質(zhì)細胞抗原2(BST2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Marrow Stromal Cell Antigen 2 (BST2)48T/96TY13057Homo sapiens (Human)
膜鐵轉(zhuǎn)運蛋白(FPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ferroportin (FPN)48T/96TY13058Mus musculus (Mouse)
Ⅳ型膠原α3(COL4α3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type IV Alpha 3 (COL4a3)48T/96TY13059Homo sapiens (Human)
原鈣黏素15(PCDH15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protocadherin 15 (PCDH15)48T/96TY13060Homo sapiens (Human)
銅伴侶超氧化物歧化酶(SOD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Superoxide Dismutase, Copper Chaperone (SOD4)48T/96TY13061Rattus norvegicus (Rat)
過氧化還原酶1(PRDX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxiredoxin 1 (PRDX1)48T/96TY13062Homo sapiens (Human)
外生骨疣樣蛋白1(EXTL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Exostoses Like Protein 1 (EXTL1)48T/96TY13063Homo sapiens (Human)
RAD23同源物B(RAD23B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for RAD23 Homolog B (RAD23B)48T/96TY13064Homo sapiens (Human)
芐氯素1(BECN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beclin 1 (BECN1)48T/96TY13065Homo sapiens (Human)
催乳素誘導(dǎo)蛋白(PIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prolactin Induced Protein (PIP)48T/96TY13066Homo sapiens (Human)
R-脊椎蛋白3(RSPO3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for R-Spondin 3 (RSPO3)48T/96TY13067Homo sapiens (Human)
干擾素α21(IFNα21)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Alpha 21 (IFNa21)48T/96TY13068Homo sapiens (Human)
唾液酸乙酰酯酶(SIAE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sialic Acid Acetylesterase (SIAE)48T/96TY13069Homo sapiens (Human)
音猬因子(SHH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hedgehog Homolog, Sonic (SHH)48T/96TY13070Rattus norvegicus (Rat)
印度刺猬因子(IHH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hedgehog Homolog, Indian (IHH)48T/96TY13071Rattus norvegicus (Rat)
胰島素樣蛋白6(INSL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Protein 6 (INSL6)48T/96TY13072Mus musculus (Mouse)
尿皮質(zhì)素2(UCN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Urocortin 2 (UCN2)48T/96TY13073Rattus norvegicus (Rat)
尿皮質(zhì)素3(UCN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Urocortin 3 (UCN3)48T/96TY13074Rattus norvegicus (Rat)
組織蛋白酶G(CTSG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin G (CTSG)48T/96TY13075Mus musculus (Mouse)
膜聯(lián)蛋白A1(ANXA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Annexin A1 (ANXA1)48T/96TY13076Mus musculus (Mouse)
白蛋白啟動子D位點結(jié)合蛋白(DBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for D Site Of Albumin Promoter Binding Protein (DBP)48T/96TY13077Rattus norvegicus (Rat)
V-Yes-1 Yamaguchi肉瘤病毒相關(guān)癌基因同源物(LYN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN)48T/96TY13078Homo sapiens (Human)
神經(jīng)生長因子誘導(dǎo)蛋白B(NGFIB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nerve Growth Factor IB (NGFIB)48T/96TY13079Rattus norvegicus (Rat)
生長因子受體結(jié)合蛋白2(Grb2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Factor Receptor Bound Protein 2 (Grb2)48T/96TY13080Homo sapiens (Human)
毛狀樣蛋白1(COTL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coactosin Like Protein 1 (COTL1)48T/96TY13081Homo sapiens (Human)
腓骨蛋白7(FBLN7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibulin 7 (FBLN7)48T/96TY13082Mus musculus (Mouse)
溶質(zhì)載體家族1成員5(SLC1A5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Solute Carrier Family 1, Member 5 (SLC1A5)48T/96TY13083Homo sapiens (Human)
P物質(zhì)(SP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Substance P (SP)48T/96TY13084Canis familiaris; Canine (Dog)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。