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小鼠c-fos ELISA檢測試劑盒 ,英文名:小鼠c-fos ELISA試劑盒 ,英文縮寫:小鼠c-fos ELISA試劑盒 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠c-fos ELISA試劑盒 小鼠c-fosELISA試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠c-fos ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
血清淀粉樣P物質(zhì)(SAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serum Amyloid P Component (SAP)48T/96TY12985Rattus norvegicus (Rat)
粒細胞趨化蛋白2(GCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Granulocyte Chemotactic Protein 2 (GCP2)48T/96TY12986Rattus norvegicus (Rat)
胃動蛋白2(GKN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gastrokine 2 (GKN2)48T/96TY12987Rattus norvegicus (Rat)
血管生成素樣蛋白7(ANGPTL7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin Like Protein 7 (ANGPTL7)48T/96TY12988Mus musculus (Mouse)
γ-氨基丁酸A受體α2(γABRα2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gamma-Aminobutyric Acid A Receptor Alpha 2 (gABRa2)48T/96TY12989Mus musculus (Mouse)
自身免疫調(diào)節(jié)因子(AIRE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Autoimmune Regulator (AIRE)48T/96TY12990Mus musculus (Mouse)
心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)48T/96TY12991Rattus norvegicus (Rat)
絨毛膜促性腺激素α肽(CGα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chorionic Gonadotropin Alpha Polypeptide (CGa)48T/96TY12992Homo sapiens (Human)
刺猬因子相互作用蛋白(HHIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hedgehog Interacting Protein (HHIP)48T/96TY12993Homo sapiens (Human)
糖基化磷脂酰肌醇特異性磷脂酶D1(GPLD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1)48T/96TY12994Homo sapiens (Human)
免疫球蛋白A1(IgA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin A1 (IgA1)48T/96TY12995Homo sapiens (Human)
免疫球蛋白A2(IgA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin A2 (IgA2)48T/96TY12996Homo sapiens (Human)
D-氨基酸氧化酶激活因子(DAOA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for D-amino Acid Oxidase Activator (DAOA)48T/96TY12997Homo sapiens (Human)
無翅型MMTV整合位點家族成員5A(WNT5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)48T/96TY12998Rattus norvegicus (Rat)
Discs大同源物4(DLG4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Discs, Large Homolog 4 (DLG4)48T/96TY12999Mus musculus (Mouse)
神經(jīng)調(diào)節(jié)素4(NRG4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuregulin 4 (NRG4)48T/96TY13000Homo sapiens (Human)
鳥氨酸氨基甲酰轉(zhuǎn)移酶(OCT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ornithine Carbamoyl Transferase (OCT)48T/96TY13001Rattus norvegicus (Rat)
神經(jīng)調(diào)節(jié)素3(NRG3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuregulin 3 (NRG3)48T/96TY13002Homo sapiens (Human)
無翅型MMTV整合位點家族成員5A(WNT5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)48T/96TY13003Mus musculus (Mouse)
Ⅰ型膠原α1(COL1α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type I Alpha 1 (COL1a1)48T/96TY13004Mus musculus (Mouse)
甘露糖受體C1(MRC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Receptor C Type 1 (MRC1)48T/96TY13005Mus musculus (Mouse)
甘露糖受體C1(MRC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Receptor C Type 1 (MRC1)48T/96TY13006Rattus norvegicus (Rat)
胰島素樣蛋白3(INSL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Protein 3 (INSL3)48T/96TY13007Homo sapiens (Human)
線粒體轉(zhuǎn)錄因子A(TFAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transcription Factor A, Mitochondrial (TFAM)48T/96TY13008Homo sapiens (Human)
CD19分子(CD19)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 19 (CD19)48T/96TY13009Homo sapiens (Human)
唾液酸結合Ig樣凝集素2(SIGLEC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sialic Acid Binding Ig Like Lectin 2 (SIGLEC2)48T/96TY13010Homo sapiens (Human)
胰高血糖素(GC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucagon (GC)48T/96TY13011Rattus norvegicus (Rat)
B-細胞激活因子(BAFF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for B-Cell Activating Factor (BAFF)48T/96TY13012Rattus norvegicus (Rat)
載脂蛋白A4(APOA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein A4 (APOA4)48T/96TY13013Mus musculus (Mouse)
封閉蛋白3(CLDN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Claudin 3 (CLDN3)48T/96TY13014Homo sapiens (Human)
磷脂酰肌醇蛋白聚糖1(GPC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glypican 1 (GPC1)48T/96TY13015Mus musculus (Mouse)
果糖二磷酸醛縮酶C(ALDOC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldolase C, Fructose Bisphosphate (ALDOC)48T/96TY13016Homo sapiens (Human)
雙特異性磷酸酶5(DUSP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dual Specificity Phosphatase 5 (DUSP5)48T/96TY13017Mus musculus (Mouse)
高遷移率族AT Hook蛋白1(HMGA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for High Mobility Group AT Hook Protein 1 (HMGA1)48T/96TY13018Homo sapiens (Human)
高遷移率族AT Hook蛋白2(HMGA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for High Mobility Group AT Hook Protein 2 (HMGA2)48T/96TY13019Homo sapiens (Human)
己糖激酶2(HK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hexokinase 2 (HK2)48T/96TY13020Homo sapiens (Human)
蛋白酪氨酸磷酸酶ⅣA3(PTPⅣA3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase Type IVA 3 (PTP4A3)48T/96TY13021Homo sapiens (Human)
CREB調(diào)節(jié)轉(zhuǎn)錄輔激活因子3(CRTC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for CREB Regulated Transcription Coactivator 3 (CRTC3)48T/96TY13022Homo sapiens (Human)
環(huán)指蛋白39(RNF39)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ring Finger Protein 39 (RNF39)48T/96TY13023Homo sapiens (Human)
組胺H4受體(HRH4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histamine Receptor H4 (HRH4)48T/96TY13024Homo sapiens (Human)
組胺H4受體(HRH4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Histamine Receptor H4 (HRH4)48T/96TY13025Mus musculus (Mouse)
含鋅飄帶域蛋白1(ZNRD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Zinc Ribbon Domain Containing Protein 1 (ZNRD1)48T/96TY13026Homo sapiens (Human)
肽酶抑制因子16(PI16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peptidase Inhibitor 16 (PI16)48T/96TY13027Homo sapiens (Human)
肌營養(yǎng)不良蛋白(DMD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dystrophin (DMD)48T/96TY13028Mus musculus (Mouse)
血小板反應蛋白解整合素金屬肽酶7(ADAMTS7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)48T/96TY13029Rattus norvegicus (Rat)
序列相似家族3成員B(FAM3B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Family With Sequence Similarity 3, Member B (FAM3B)48T/96TY13030Homo sapiens (Human)
Slit同源物3(Slit3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Slit Homolog 3 (Slit3)48T/96TY13031Mus musculus (Mouse)
Slit同源物3(Slit3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Slit Homolog 3 (Slit3)48T/96TY13032Homo sapiens (Human)
白介素18受體1(IL18R1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 Receptor 1 (IL18R1)48T/96TY13033Homo sapiens (Human)
白介素18受體輔助蛋白(IL18RAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 Receptor Accessory Protein (IL18RAP)48T/96TY13034Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。