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小鼠肌鈣蛋白T酶免試劑盒,(Tn-T)ELISA檢測試劑盒 ,英文名:Tn-T ELISA Kit ,英文縮寫:Tn-T ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠肌鈣蛋白ISA檢測試劑盒,Tn-T 檢測試劑盒, Tn-T 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠肌鈣蛋白T酶免試劑盒,(Tn-T)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
RAS癌基因家族成員RAB37(RAB37)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for RAB37, Member RAS Oncogene Family (RAB37)48T/96TY12935Homo sapiens (Human)
RIO激酶1(RIOK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for RIO Kinase 1 (RIOK1)48T/96TY12936Homo sapiens (Human)
惡性腦腫瘤缺失蛋白1(DMBT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Deleted In Malignant Brain Tumors 1 (DMBT1)48T/96TY12937Homo sapiens (Human)
鈣調(diào)蛋白樣蛋白3(CALML3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calmodulin Like Protein 3 (CALML3)48T/96TY12938Homo sapiens (Human)
白介素1α(IL1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY12939Bos taurus; Bovine (Cattle)
原卟啉原氧化酶(PPOX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protoporphyrinogen Oxidase (PPOX)48T/96TY12940Homo sapiens (Human)
原卟啉原氧化酶(PPOX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protoporphyrinogen Oxidase (PPOX)48T/96TY12941Rattus norvegicus (Rat)
鳥氨酸脫羧酶抗酶1(OAZ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ornithine Decarboxylase Antizyme 1 (OAZ1)48T/96TY12942Homo sapiens (Human)
再生胰島衍生蛋白3α(REG3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 3 Alpha (REG3a)48T/96TY12943Homo sapiens (Human)
橋粒芯膠粘蛋白3(DSC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Desmocollin 3 (DSC3)48T/96TY12944Homo sapiens (Human)
表皮膜蛋白3(EMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Epithelial Membrane Protein 3 (EMP3)48T/96TY12945Homo sapiens (Human)
半乳糖-1-磷酸尿苷酰轉(zhuǎn)移酶(GALT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Galactose-1-Phosphate Uridylyltransferase (GALT)48T/96TY12946Rattus norvegicus (Rat)
腫瘤壞死因子受體超家族成員10A(TNFRSF10A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Receptor Superfamily, Member 10A (TNFRSF10A)48T/96TY12947Mus musculus (Mouse)
血清淀粉樣蛋白A2(SAA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serum Amyloid A2 (SAA2)48T/96TY12948Mus musculus (Mouse)
磷酸二酯酶12(PDE12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphodiesterase 12 (PDE12)48T/96TY12949Mus musculus (Mouse)
S100鈣結(jié)合蛋白A14(S100A14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A14 (S100A14)48T/96TY12950Homo sapiens (Human)
胃蛋白酶原A(PGA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pepsinogen A (PGA)48T/96TY12951Rattus norvegicus (Rat)
泛素羧基端酯酶L1(UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)48T/96TY12952Rattus norvegicus (Rat)
嗜酸性粒細(xì)胞過氧化物酶(EPX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Eosinophil Peroxidase (EPX)48T/96TY12953Homo sapiens (Human)
蛋白聚糖4(PRG4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proteoglycan 4 (PRG4)48T/96TY12954Homo sapiens (Human)
X-連鎖凋亡蛋白抑制因子(XIAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for X-Linked Inhibitor Of Apoptosis Protein (XIAP)48T/96TY12955Homo sapiens (Human)
血小板衍生生長因子C(PDGFC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor C (PDGFC)48T/96TY12956Mus musculus (Mouse)
泛素特異性肽酶8(USP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ubiquitin Specific Peptidase 8 (USP8)48T/96TY12957Homo sapiens (Human)
醌NADH脫氫酶1(NQO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for NADH Dehydrogenase, Quinone 1 (NQO1)48T/96TY12958Homo sapiens (Human)
醌NADH脫氫酶1(NQO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for NADH Dehydrogenase, Quinone 1 (NQO1)48T/96TY12959Mus musculus (Mouse)
和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Copeptin (CPP)48T/96TY12960Mus musculus (Mouse)
白介素1β(IL1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY12961Equus caballus; Equine (Horse)
載脂蛋白L1(APOL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein L (APOL1)48T/96TY12962Homo sapiens (Human)
四旋蛋白30(TSPAN30)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tetraspanin 30 (TSPAN30)48T/96TY12963Homo sapiens (Human)
粘蛋白13(MUC13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucin 13, Cell Surface Associated (MUC13)48T/96TY12964Homo sapiens (Human)
RAD54樣蛋白2(RAD54L2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for RAD54 Like Protein 2 (RAD54L2)48T/96TY12965Homo sapiens (Human)
S100鈣結(jié)合蛋白A4(S100A4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A4 (S100A4)48T/96TY12966Rattus norvegicus (Rat)
亨廷頓蛋白(HTT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Huntingtin (HTT)48T/96TY12967Homo sapiens (Human)
染色質(zhì)域解旋酶DNA結(jié)合蛋白4(CHD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chromodomain Helicase DNA Binding Protein 4 (CHD4)48T/96TY12968Mus musculus (Mouse)
R-脊椎蛋白1(RSPO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for R-Spondin 1 (RSPO1)48T/96TY12969Mus musculus (Mouse)
肌營養(yǎng)不良蛋白關(guān)聯(lián)糖蛋白1(DAG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dystrophin Associated Glycoprotein 1 (DAG1)48T/96TY12970Homo sapiens (Human)
雙糖鏈蛋白聚糖(BGN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Biglycan (BGN)48T/96TY12971Homo sapiens (Human)
組織蛋白酶D(CTSD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin D (CTSD)48T/96TY12972Bos taurus; Bovine (Cattle)
組織蛋白酶D(CTSD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin D (CTSD)48T/96TY12973Rattus norvegicus (Rat)
間皮素(MSLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mesothelin (MSLN)48T/96TY12974Mus musculus (Mouse)
突觸極蛋白2(SYNPO2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Synaptopodin 2 (SYNPO2)48T/96TY12975Mus musculus (Mouse)
角膜蛋白(KERA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratocan (KERA)48T/96TY12976Homo sapiens (Human)
正五聚蛋白3(PTX3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pentraxin 3, Long (PTX3)48T/96TY12977Rattus norvegicus (Rat)
中間α-球蛋白抑制因子H5(ITIH5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inter Alpha-Globulin Inhibitor H5 (ITIH5)48T/96TY12978Homo sapiens (Human)
中間α-球蛋白抑制因子H2(ITIH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inter Alpha-Globulin Inhibitor H2 (ITIH2)48T/96TY12979Homo sapiens (Human)
中間α-球蛋白抑制因子H1(ITIH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inter Alpha-Globulin Inhibitor H1 (ITIH1)48T/96TY12980Homo sapiens (Human)
CD72分子(CD72)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 72 (CD72)48T/96TY12981Homo sapiens (Human)
血漿谷胱甘肽過氧化物酶(GPX3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione Peroxidase 3, Plasma (GPX3)48T/96TY12982Homo sapiens (Human)
神經(jīng)軟骨蛋白(NCDN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neurochondrin (NCDN)48T/96TY12983Rattus norvegicus (Rat)
前蛋白轉(zhuǎn)化酶枯草溶菌素5(PCSK5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proprotein Convertase Subtilisin/Kexin Type 5 (PCSK5)48T/96TY12984Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。