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小鼠抗核抗體酶免試劑盒,(ANA)ELISA檢測試劑盒 ,英文名:ANA ELISA Kit ,英文縮寫:ANA ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠抗核抗體ELISA檢測試劑盒,ANA 檢測試劑盒, ANA 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠抗核抗體酶免試劑盒,(ANA)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復(fù)性。
分揀蛋白1(SORT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sortilin 1 (SORT1)48T/96TY12685Homo sapiens (Human)
淀粉樣蛋白β前體樣蛋白2(APLP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amyloid Beta Precursor Like Protein 2 (APLP2)48T/96TY12686Homo sapiens (Human)
鈣離子轉(zhuǎn)運ATP酶A2(ATP2A2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)48T/96TY12687Homo sapiens (Human)
尿調(diào)蛋白(UMOD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Uromodulin (UMOD)48T/96TY12688Rattus norvegicus (Rat)
S100鈣結(jié)合蛋白A8(S100A8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)48T/96TY12689Mus musculus (Mouse)
S100鈣結(jié)合蛋白A9(S100A9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A9 (S100A9)48T/96TY12690Mus musculus (Mouse)
蛋白Z依賴性蛋白酶抑制因子(ZPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Z Dependent Protease Inhibitor (ZPI)48T/96TY12691Homo sapiens (Human)
賴氨酰氧化酶(LOX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysyl Oxidase (LOX)48T/96TY12692Homo sapiens (Human)
胞裂蛋白6(SEPT6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Septin 6 (SEPT6)48T/96TY12693Homo sapiens (Human)
蛋氨酸腺苷轉(zhuǎn)移酶Ⅱα(MAT2α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Methionine Adenosyltransferase II Alpha (MAT2a)48T/96TY12694Homo sapiens (Human)
GPI錨定分子樣蛋白(GML)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycosylphosphatidylinositol Anchored Molecule Like Protein (GML)48T/96TY12695Homo sapiens (Human)
B-細胞κ-輕肽基因增強子抑制因子激酶β(IκBKβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb)48T/96TY12696Mus musculus (Mouse)
核糖體蛋白L23A(RPL23A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ribosomal Protein L23A (RPL23A)48T/96TY12697Homo sapiens (Human)
同線蛋白2(ST2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Syntenin 2 (ST2)48T/96TY12698Homo sapiens (Human)
Endoglin蛋白(ENG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endoglin (ENG)48T/96TY12699Canis familiaris; Canine (Dog)
CD19分子(CD19)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 19 (CD19)48T/96TY12700Rattus norvegicus (Rat)
鉀中間小電導(dǎo)鈣激活通道蛋白亞家族N成員2(KCNN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2)48T/96TY12701Homo sapiens (Human)
白介素1家族成員9(IL1F9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Family, Member 9 (IL1F9)48T/96TY12702Homo sapiens (Human)
增殖關(guān)聯(lián)蛋白2G4(PA2G4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proliferation Associated Protein 2G4 (PA2G4)48T/96TY12703Homo sapiens (Human)
類固醇5α還原酶1(SRD5α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Steroid 5 Alpha Reductase 1 (SRD5a1)48T/96TY12704Rattus norvegicus (Rat)
膜聯(lián)蛋白A4(ANXA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Annexin A4 (ANXA4)48T/96TY12705Homo sapiens (Human)
角蛋白9(KRT9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 9 (KRT9)48T/96TY12706Mus musculus (Mouse)
多功能蛋白聚糖(VS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Versican (VS)48T/96TY12707Mus musculus (Mouse)
密封蛋白(OCLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Occludin (OCLN)48T/96TY12708Mus musculus (Mouse)
分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Secreted Frizzled Related Protein 1 (SFRP1)48T/96TY12709Homo sapiens (Human)
運動因子相關(guān)蛋白1(MRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Motility Related Protein (MRP1)48T/96TY12710Homo sapiens (Human)
骨成型蛋白3(BMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bone Morphogenetic Protein 3 (BMP3)48T/96TY12711Homo sapiens (Human)
非受體酪氨酸激酶2(TNK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tyrosine Kinase, Non Receptor 2 (TNK2)48T/96TY12712Homo sapiens (Human)
封閉蛋白2(CLDN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Claudin 2 (CLDN2)48T/96TY12713Mus musculus (Mouse)
肌鈀蛋白(MYPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myopalladin (MYPN)48T/96TY12714Homo sapiens (Human)
血管非炎性蛋白1(VNN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vanin 1 (VNN1)48T/96TY12715Rattus norvegicus (Rat)
鳥苷酸環(huán)化酶激活因子2A(GUCA2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Guanylate Cyclase Activator 2A (GUCA2A)48T/96TY12716Homo sapiens (Human)
GM2神經(jīng)節(jié)苷脂激活因子(GM2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for GM2 Ganglioside Activator (GM2A)48T/96TY12717Homo sapiens (Human)
溶酶體酸性磷酸酶2(ACP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 2, Lysosomal (ACP2)48T/96TY12718Homo sapiens (Human)
溶酶體酸性磷酸酶2(ACP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Acid Phosphatase 2, Lysosomal (ACP2)48T/96TY12719Mus musculus (Mouse)
組織蛋白酶D(CTSD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin D (CTSD)48T/96TY12720Homo sapiens (Human)
組織蛋白酶D(CTSD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin D (CTSD)48T/96TY12721Mus musculus (Mouse)
精氨酸酶(Arg)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arginase (Arg)48T/96TY12722Mus musculus (Mouse)
Discs大同源物4(DLG4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Discs, Large Homolog 4 (DLG4)48T/96TY12723Rattus norvegicus (Rat)
中性鞘磷脂酶(NSMASE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neutral Sphingomyelinase (NSMASE)48T/96TY12724Homo sapiens (Human)
抑制素βE(INHβE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibin Beta E (INHbE)48T/96TY12725Homo sapiens (Human)
DEAD框肽5(DDX5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for DEAD Box Polypeptide 5 (DDX5)48T/96TY12726Homo sapiens (Human)
乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoferrin (LTF)48T/96TY12727Sus scrofa; Porcine (Pig)
蛋白二硫化物異構(gòu)酶A2(PDIA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Disulfide Isomerase A2 (PDIA2)48T/96TY12728Mus musculus (Mouse)
蛋白二硫化物異構(gòu)酶A5(PDIA5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Disulfide Isomerase A5 (PDIA5)48T/96TY12729Homo sapiens (Human)
核小體裝配蛋白1樣1(NAP1L1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1)48T/96TY12730Homo sapiens (Human)
妊娠帶蛋白(PZP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pregnancy Zone Protein (PZP)48T/96TY12731Homo sapiens (Human)
精氨酸/絲氨酸豐富剪接因子2(SRSF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serine/Arginine Rich Splicing Factor 2 (SRSF2)48T/96TY12732Homo sapiens (Human)
醛脫氫酶1家族成員A1(ALDH1A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)48T/96TY12733Homo sapiens (Human)
前列腺蛋白類脂肪酶B(LIPB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipophilin B, Prostatein Like (LIPB)48T/96TY12734Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。