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小鼠基質金屬蛋白酶2/明膠酶A酶免試劑盒,(MMP-2/Gelatinase A)ELISA檢測試劑盒,英文名:MMP-2/Gelatinase A ELISA Kit,英文縮寫:MMP-2/Gelatinase A ,常見試劑盒規(guī)格:96T/48T,產品別名:小鼠基質金屬蛋白酶2/明膠酶AELISA檢測試劑盒,MMP-2/Gelatinase A 檢測試劑盒,MMP-2/Gelatinase A 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠基質金屬蛋白酶2/明膠酶A酶免試劑盒,(MMP-2/Gelatinase A)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
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胰島素樣生長因子結合蛋白2(IGFBP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2)48T/96TY11549Bos taurus; Bovine (Cattle)
胰島素樣生長因子結合蛋白2(IGFBP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2)48T/96TY11550Sus scrofa; Porcine (Pig)
雙調蛋白(AREG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Amphiregulin (AREG)48T/96TY11551Oryctolagus cuniculus (Rabbit)
窖蛋白(CAV1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11552Bos taurus; Bovine (Cattle)
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窖蛋白(CAV1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11560Rhesus monkey (Simian)
轉化生長因子β誘導蛋白(TGFβI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)48T/96TY11561Bos taurus; Bovine (Cattle)
轉化生長因子β3(TGFβ3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY11562Canis familiaris; Canine (Dog)
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轉化生長因子β誘導蛋白(TGFβI)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)48T/96TY11570Oryctolagus cuniculus (Rabbit)
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組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY11572Bos taurus; Bovine (Cattle)
組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY11573Sus scrofa; Porcine (Pig)
組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY11574Oryctolagus cuniculus (Rabbit)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY11575Capra hircus; Caprine (Goat)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY11576Equus caballus; Equine (Horse)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11577Bos taurus; Bovine (Cattle)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11578Canis familiaris; Canine (Dog)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11579Sus scrofa; Porcine (Pig)
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腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11581Rhesus monkey (Simian)
胰島素樣生長因子結合蛋白3(IGFBP3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY11582Bos taurus; Bovine (Cattle)
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胰島素樣生長因子結合蛋白3(IGFBP3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY11584Equus caballus; Equine (Horse)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。