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小鼠趨化因子酶免試劑盒,(fractalkine/CX3CL1) ELISA檢測(cè)試劑盒,英文名:fractalkine/CX3CL1) ELISA試劑盒,英文縮寫(xiě):fractalkine/CX3CL1) ELISA試劑盒,常見(jiàn)試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠趨化因子ELISA檢測(cè)試劑盒,fractalkine/CX3CL1 檢測(cè)試劑盒,fractalkine/CX3CL1 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠趨化因子酶免試劑盒,(fractalkine/CX3CL1) ELISA檢測(cè)試劑盒 在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋?zhuān)螂x心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過(guò)程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無(wú)關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過(guò)程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶5(NOX5)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5 (NOX5)48T/96TY10585Oryctolagus cuniculus (Rabbit)
基質(zhì)金屬蛋白酶9(MMP9)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Matrix Metalloproteinase 9 (MMP9)48T/96TY10586Oryctolagus cuniculus (Rabbit)
甲狀旁腺激素(PTH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Parathyroid Hormone (PTH)48T/96TY10587Homo sapiens (Human)
組織金屬蛋白酶抑制因子1(TIMP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)48T/96TY10588Oryctolagus cuniculus (Rabbit)
脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2)48T/96TY10589Canis familiaris; Canine (Dog)
堿性成纖維細(xì)胞生長(zhǎng)因子(FGF2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)48T/96TY10590Oryctolagus cuniculus (Rabbit)
巨噬細(xì)胞炎性蛋白相關(guān)蛋白1(MRP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Macrophage Inflammatory Protein Related Protein 1 (MRP1)48T/96TY10591Rattus norvegicus (Rat)
內(nèi)皮型一氧化氮合酶(NOS3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY10592Canis familiaris; Canine (Dog)
甲狀腺素受體β(THRβ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Thyroid Hormone Receptor Beta (THRb)48T/96TY10593Homo sapiens (Human)
血管細(xì)胞粘附分子1(VCAM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)48T/96TY10594Oryctolagus cuniculus (Rabbit)
熱休克蛋白β2(HSPβ2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Heat Shock Protein Beta 2 (HSPb2)48T/96TY10595Chicken (Gallus)
免疫球蛋白G(IgG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY10596Oryctolagus cuniculus (Rabbit)
免疫球蛋白M(IgM)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Immunoglobulin M (IgM)48T/96TY10597Oryctolagus cuniculus (Rabbit)
內(nèi)皮素1(EDN1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10598Oryctolagus cuniculus (Rabbit)
緩激肽(BK)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Bradykinin (BK)48T/96TY10599Mus musculus (Mouse)
髓鞘堿性蛋白(MBP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myelin Basic Protein (MBP)48T/96TY10600Oryctolagus cuniculus (Rabbit)
神經(jīng)營(yíng)養(yǎng)因子4(NT4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neurotrophin 4 (NT4)48T/96TY10601Xenopus (Clawed frog)
瘦素(LEP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Leptin (LEP)48T/96TY10602Oryctolagus cuniculus (Rabbit)
抗甲狀腺微粒體抗體(ATMA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Anti-Thyroid Microsome Antibody (ATMA)48T/96TY10603Homo sapiens (Human)
細(xì)胞間粘附分子1(ICAM1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)48T/96TY10604Oryctolagus cuniculus (Rabbit)
細(xì)胞間粘附分子2(ICAM2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2)48T/96TY10605Oryctolagus cuniculus (Rabbit)
纖溶酶原激活物抑制因子1(PAI1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1)48T/96TY10606Oryctolagus cuniculus (Rabbit)
內(nèi)皮素1(EDN1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10607Rattus norvegicus (Rat)
纖溶酶原激活物抑制因子2(PAI2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plasminogen Activator Inhibitor 2 (PAI2)48T/96TY10608Oryctolagus cuniculus (Rabbit)
蛋白C(PROC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protein C (PROC)48T/96TY10609Oryctolagus cuniculus (Rabbit)
血栓調(diào)節(jié)蛋白(TM)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Thrombomodulin (TM)48T/96TY10610Oryctolagus cuniculus (Rabbit)
過(guò)氧化物酶體增殖物激活受體γ(PPARγ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma (PPARg)48T/96TY10611Oryctolagus cuniculus (Rabbit)
血小板衍生生長(zhǎng)因子A(PDGFA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plaet Derived Growth Factor Subunit A (PDGFA)48T/96TY10612Oryctolagus cuniculus (Rabbit)
補(bǔ)體成分4a(C4a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Complement Component 4a (C4a)48T/96TY10613Oryctolagus cuniculus (Rabbit)
胰島素(INS)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin (INS)48T/96TY10614Oryctolagus cuniculus (Rabbit)
胰島素樣生長(zhǎng)因子1(IGF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY10615Oryctolagus cuniculus (Rabbit)
胰島素樣生長(zhǎng)因子2(IGF2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor 2 (IGF2)48T/96TY10616Oryctolagus cuniculus (Rabbit)
腎素(REN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Renin (REN)48T/96TY10617Oryctolagus cuniculus (Rabbit)
組織因子(TF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Factor (TF)48T/96TY10618Oryctolagus cuniculus (Rabbit)
內(nèi)皮素1(EDN1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Endothelin 1 (EDN1)48T/96TY10619Homo sapiens (Human)
半胱氨酸蛋白酶抑制劑3(CST3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cystatin 3 (CST3)48T/96TY10620Oryctolagus cuniculus (Rabbit)
骨橋素(OPN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Osteopontin (OPN)48T/96TY10621Canis familiaris; Canine (Dog)
前列腺素E1(PGE1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Prostaglandin E1 (PGE1)48T/96TY10622General
前列腺素E合酶2(PTGES2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Prostaglandin E Synthase 2 (PTGES2)48T/96TY10623Homo sapiens (Human)
前列腺素E合酶2(PTGES2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Prostaglandin E Synthase 2 (PTGES2)48T/96TY10624Rattus norvegicus (Rat)
銅藍(lán)蛋白(CP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Ceruloplasmin (CP)48T/96TY10625Mus musculus (Mouse)
醛固酮(ALD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Aldosterone (ALD)48T/96TY10626General
血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY10627Homo sapiens (Human)
熱休克轉(zhuǎn)錄因子4(HSF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Heat Shock Transcription Factor 4 (HSF4)48T/96TY10628Mus musculus (Mouse)
血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY10629Mus musculus (Mouse)
血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plaet Factor 4 (PF4)48T/96TY10630Rattus norvegicus (Rat)
維生素K1(VK1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vitamin K1 (VK1)48T/96TY10631General
C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for C Reactive Protein (CRP)48T/96TY10632Sus scrofa; Porcine (Pig)
神經(jīng)肽Y(NPY)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neuropeptide Y (NPY)48T/96TY10633Homo sapiens (Human)
神經(jīng)肽Y(NPY)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neuropeptide Y (NPY)48T/96TY10634Rattus norvegicus (Rat)
神經(jīng)肽Y(NPY)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neuropeptide Y (NPY)48T/96TY10635Mus musculus (Mouse)
神經(jīng)肽Y(NPY)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neuropeptide Y (NPY)48T/96TY10636Bos taurus; Bovine (Cattle)
白介素12A(IL12A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 12A (IL12A)48T/96TY10637Chicken (Gallus)
載脂蛋白B(APOB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Apolipoprotein B (APOB)48T/96TY10638Sus scrofa; Porcine (Pig)
細(xì)胞絨毛蛋白(CVL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cytovillin (CVL)48T/96TY10639Rattus norvegicus (Rat)
大型中性氨基酸轉(zhuǎn)運(yùn)蛋白1(LAT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Large Neutral Amino Acid Transporter 1 (LAT1)48T/96TY10640Homo sapiens (Human)
白介素8受體β(IL8Rβ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 8 Receptor Beta (IL8Rb)48T/96TY10641Rattus norvegicus (Rat)
白介素34(IL34)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 34 (IL34)48T/96TY10642Homo sapiens (Human)
白介素34(IL34)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 34 (IL34)48T/96TY10643Mus musculus (Mouse)
強(qiáng)啡肽(Dyn)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Big Dynorphin (Dyn)48T/96TY10644Mus musculus (Mouse)
腦啡肽(ENK)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Enkephalin (ENK)48T/96TY10645Mus musculus (Mouse)
成纖維生長(zhǎng)因子受體底物2(FRS2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor Receptor Substrate 2 (FRS2)48T/96TY10646Rattus norvegicus (Rat)
白細(xì)胞介素24(IL24)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 24 (IL24)48T/96TY10647Homo sapiens (Human)
金屬硫蛋白1(MT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Metallothionein 1 (MT1)48T/96TY10648Mus musculus (Mouse)
肌肉生長(zhǎng)抑制素(MSTN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Myostatin (MSTN)48T/96TY10649Mus musculus (Mouse)
碳酸酐酶Ⅱ(CA2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Carbonic Anhydrase II (CA2)48T/96TY10650Chicken (Gallus)
雨蛙素(INN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Ceruletide (INN)48T/96TY10651General
α2-HS糖蛋白(αHSG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG)48T/96TY10652Mus musculus (Mouse)
核因子κB受體激活因子配基(RANκL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)48T/96TY10653Chicken (Gallus)
早期生長(zhǎng)應(yīng)答因子4(EGR4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Early Growth Response Protein 4 (EGR4)48T/96TY10654Mus musculus (Mouse)
葡萄糖6磷酸脫氫酶(G6PD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glucose 6 Phosphate Dehydrogenase (G6PD)48T/96TY10655Homo sapiens (Human)
葡萄糖6磷酸脫氫酶(G6PD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glucose 6 Phosphate Dehydrogenase (G6PD)48T/96TY10656Rattus norvegicus (Rat)
谷氨酸脫羧酶2(GAD2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glutamate Decarboxylase 2, Acid (GAD2)48T/96TY10657Rattus norvegicus (Rat)
谷氨酸脫羧酶2(GAD2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glutamate Decarboxylase 2, Acid (GAD2)48T/96TY10658Mus musculus (Mouse)
山梨醇脫氫酶(SDH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Sorbitol Dehydrogenase (SDH)48T/96TY10659Rattus norvegicus (Rat)
硫氧還蛋白結(jié)合蛋白2(TBP2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Thioredoxin Binding Protein 2 (TBP2)48T/96TY10660Mus musculus (Mouse)
細(xì)胞色素P450家族成員7A1(CYP7A1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cytochrome P450 7A1 (CYP7A1)48T/96TY10661Homo sapiens (Human)
血紅素結(jié)合蛋白(HPX)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Hemopexin (HPX)48T/96TY10662Rattus norvegicus (Rat)
谷丙轉(zhuǎn)氨酶(ALT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) ELISA Kit for Alanine Aminotransferase (ALT)48T/96TY10663Cavia (Guinea pig )
谷丙轉(zhuǎn)氨酶(ALT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alanine Aminotransferase (ALT)48T/96TY10664Homo sapiens (Human)
谷丙轉(zhuǎn)氨酶(ALT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alanine Aminotransferase (ALT)48T/96TY10665Sus scrofa; Porcine (Pig)
各種類(lèi)型ELISA測(cè)定時(shí), 一般需經(jīng)過(guò)以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。