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人抗流行性出血熱病毒抗體IgG 酶免試劑盒,(EHF)ELISA檢測試劑盒,英文名:EHF ELISA Kit,英文縮寫:EHF ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗流行性出血熱病毒抗體IgG ELISA檢測試劑盒,EHF 檢測試劑盒,EHF 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗流行性出血熱病毒抗體IgG 酶免試劑盒,(EHF)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競爭法及定量檢測影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
磷脂酰肌醇特異性磷酯酶Cβ1(PLCβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phospholipase C Beta 1, Phosphoinositide Specific (PLCb1)48T/96TY09685Homo sapiens (Human)
細(xì)胞附著蛋白4(CYTH4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cytohesin 4 (CYTH4)48T/96TY09686Homo sapiens (Human)
干擾素調(diào)節(jié)因子5(IRF5)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Regulatory Factor 5 (IRF5)48T/96TY09687Homo sapiens (Human)
細(xì)胞附著蛋白相互作用蛋白(CYTIP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cytohesin Interacting Protein (CYTIP)48T/96TY09688Homo sapiens (Human)
B-細(xì)胞連接蛋白(BLNK)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for B-Cell Linker Protein (BLNK)48T/96TY09689Homo sapiens (Human)
B-淋巴細(xì)胞趨化因子1(BLC1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for B-Lymphocyte Chemoattractant 1 (BLC1)48T/96TY09690Homo sapiens (Human)
生長調(diào)節(jié)致癌基因β(GROβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Growth Regulated Oncogene Beta (GROb)48T/96TY09691Homo sapiens (Human)
生長調(diào)節(jié)致癌基因γ(GROγ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Growth Regulated Oncogene Gamma (GROg)48T/96TY09692Homo sapiens (Human)
磷脂酰肌醇特異性磷酯酶Cβ3(PLCβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phospholipase C Beta 3, Phosphoinositide Specific (PLCb3)48T/96TY09693Homo sapiens (Human)
糖磷脂酰肌醇(GPI)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glycophosphatidylinositol (GPI)48T/96TY09694Homo sapiens (Human)
胸腎表達(dá)趨化因子(BRAK)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Breast And Kidney Expressed Chemokine (BRAK)48T/96TY09695Homo sapiens (Human)
皮膚T-細(xì)胞虜獲趨化因子(CTACK)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cutaneous T-Cell Attracting Chemokine (CTACK)48T/96TY09696Homo sapiens (Human)
D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for D-Dimer (D2D)48T/96TY09697Oryctolagus cuniculus (Rabbit)
抑制素βC(INHβC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Inhibin Beta C (INHbC)48T/96TY09698Homo sapiens (Human)
Endoglin蛋白(ENG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Endoglin (ENG)48T/96TY09699Homo sapiens (Human)
肝脂酶(LIPC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lipase, Hepatic (LIPC)48T/96TY09700Homo sapiens (Human)
肝脂酶(LIPC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lipase, Hepatic (LIPC)48T/96TY09701Rattus norvegicus (Rat)
肝脂酶(LIPC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lipase, Hepatic (LIPC)48T/96TY09702Mus musculus (Mouse)
鈣/鈣調(diào)蛋白依賴性蛋白激酶Ⅱγ(CAMK2γ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g)48T/96TY09703Rattus norvegicus (Rat)
血管細(xì)胞粘附分子1(VCAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)48T/96TY09704Rhesus monkey (Simian)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY09705Rhesus monkey (Simian)
干擾素α(IFNα)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY09706Rhesus monkey (Simian)
α-內(nèi)啡肽(αEP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Alpha-Endorphin (aEP)48T/96TY09707Homo sapiens (Human)
單核細(xì)胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1)48T/96TY09708Rhesus monkey (Simian)
巨噬細(xì)胞移動(dòng)抑制因子(MIF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Macrophage Migration Inhibitory Factor (MIF)48T/96TY09709Rhesus monkey (Simian)
胰島素(INS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin (INS)48T/96TY09710Rhesus monkey (Simian)
金屬硫蛋白1(MT1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Metallothionein 1 (MT1)48T/96TY09711Bos taurus; Bovine (Cattle)
干擾素γ(IFNγ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY09712Rhesus monkey (Simian)
白介素6(IL6)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 6 (IL6)48T/96TY09713Rhesus monkey (Simian)
磷酸烯醇式丙酮酸羧激酶1(PCK1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)48T/96TY09714Mus musculus (Mouse)
腎上腺髓質(zhì)素(ADM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Adrenomedullin (ADM)48T/96TY09715Rattus norvegicus (Rat)
腎上腺髓質(zhì)素(ADM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Adrenomedullin (ADM)48T/96TY09716Mus musculus (Mouse)
白介素2受體β(IL2Rβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 2 Receptor Beta (IL2Rb)48T/96TY09717Homo sapiens (Human)
白介素2受體β(IL2Rβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 2 Receptor Beta (IL2Rb)48T/96TY09718Rattus norvegicus (Rat)
白介素2受體β(IL2Rβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 2 Receptor Beta (IL2Rb)48T/96TY09719Mus musculus (Mouse)
轉(zhuǎn)錄因子20(TCF20)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transcription factor 20 (TCF20)48T/96TY09720Mus musculus (Mouse)
熱休克轉(zhuǎn)錄因子1(HSF1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Heat Shock Transcription Factor 1 (HSF1)48T/96TY09721Homo sapiens (Human)
熱休克轉(zhuǎn)錄因子1(HSF1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Heat Shock Transcription Factor 1 (HSF1)48T/96TY09722Mus musculus (Mouse)
白介素18(IL18)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 18 (IL18)48T/96TY09723Chicken (Gallus)
干擾素α(IFNα)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY09724Chicken (Gallus)
抗載脂蛋白抗體(AAHA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Anti-Apolipoprotein Antibodies (AAHA)48T/96TY09725Homo sapiens (Human)
淋巴細(xì)胞胞漿蛋白2(LCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Lymphocyte Cytosolic Protein 2 (LCP2)48T/96TY09726Homo sapiens (Human)
T-細(xì)胞激活連接蛋白(LAT)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Linker For Activation Of T-Cell (LAT)48T/96TY09727Homo sapiens (Human)
蛋白酪氨酸磷酸酶受體H(PTPRH)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protein Tyrosine Phosphatase Receptor Type H (PTPRH)48T/96TY09728Homo sapiens (Human)
神經(jīng)氨酸酶(NEU)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neuraminidase (NEU)48T/96TY09729Homo sapiens (Human)
原鈣黏素α1(PCDHα1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protocadherin Alpha 1 (PCDHa1)48T/96TY09730Homo sapiens (Human)
磷酯酶Cγ1(PLCγ1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Phospholipase C Gamma 1 (PLCg1)48T/96TY09731Homo sapiens (Human)
Ras GTP酶激活蛋白1(RASA1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Ras GTPase Activating Protein 1 (RASA1)48T/96TY09732Homo sapiens (Human)
GDP解離抑制因子1(GDI1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for GDP Dissociation Inhibitor 1 (GDI1)48T/96T Y09733Homo sapiens (Human)
二胺氧化酶(DAO)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Diamine Oxidase (DAO)48T/96TY09734Mus musculus (Mouse)
各種類型ELISA測定時(shí), 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。