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人抗丙型肝炎病毒抗體酶免試劑盒,(anti-HCV)ELISA檢測試劑盒 ,英文名:anti-HCV ELISA Kit ,英文縮寫:anti-HCV ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗丙型肝炎病毒抗體ELISA檢測試劑盒,anti-HCV 檢測試劑盒, anti-HCV 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。人抗丙型肝炎病毒抗體酶免試劑盒,(anti-HCV)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結(jié)合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
心磷脂合酶(CLS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cardiolipin Synthase (CLS)48T/96TY09585Homo sapiens (Human)
腎上腺髓質(zhì)素(ADM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adrenomedullin (ADM)48T/96TY09586Canis familiaris; Canine (Dog)
白介素2受體β(IL2Rβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 2 Receptor Beta (IL2Rb)48T/96TY09587Rhesus monkey (Simian)
轉(zhuǎn)錄因子20(TCF20)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transcription factor 20 (TCF20)48T/96TY09588Homo sapiens (Human)
熱休克轉(zhuǎn)錄因子1(HSF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Transcription Factor 1 (HSF1)48T/96TY09589Rattus norvegicus (Rat)
白介素18(IL18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 (IL18)48T/96TY09590Canis familiaris; Canine (Dog)
干擾素α(IFNα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY09591Canis familiaris; Canine (Dog)
蛋白酪氨酸磷酸酶受體H(PTPRH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase Receptor Type H (PTPRH)48T/96TY09592Mus musculus (Mouse)
2,3-二磷酸甘油酸(2,3-BPG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 2,3-Bisphosphoglycerate (2,3-BPG)48T/96TY09593General
CCAAT增強子結(jié)合蛋白β(CEBPβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for CCAAT/Enhancer Binding Protein Beta (CEBPb)48T/96TY09594Homo sapiens (Human)
Toll樣受體9(TLR9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 9 (TLR9)48T/96TY09595Rattus norvegicus (Rat)
Toll樣受體9(TLR9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 9 (TLR9)48T/96TY09596Mus musculus (Mouse)
生長激素(GH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Hormone (GH)48T/96TY09597Chicken (Gallus)
胰島素樣生長因子1(IGF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY09598Chicken (Gallus)
堿性成纖維細胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)48T/96TY09599Chicken (Gallus)
免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G (IgG)48T/96TY09600Chicken (Gallus)
血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)48T/96TY09601Chicken (Gallus)
C反應蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C Reactive Protein (CRP)48T/96TY09602Chicken (Gallus)
轉(zhuǎn)化生長因子β1(TGFβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)48T/96TY09603Chicken (Gallus)
白血病抑制因子(LIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY09604Chicken (Gallus)
白介素3(IL3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 3 (IL3)48T/96TY09605Chicken (Gallus)
白介素9(IL9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 9 (IL9)48T/96TY09606Chicken (Gallus)
白介素19(IL19)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 19 (IL19)48T/96TY09607Rattus norvegicus (Rat)
補體受體2(CR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Receptor 2 (CR2)48T/96TY09608Homo sapiens (Human)
環(huán)腺苷二磷酸核糖水解酶(cADPRH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclic ADP Ribose Hydrolase (cADPRH)48T/96TY09609Homo sapiens (Human)
突觸核蛋白γ(γSYN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Synuclein Gamma (SNCg)48T/96TY09610Homo sapiens (Human)
腎足蛋白(NPHN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nephrin (NPHN)48T/96TY09611Homo sapiens (Human)
Podocin蛋白(PDCN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Podocin (PDCN)48T/96TY09612Homo sapiens (Human)
肌紅蛋白(MYO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myoglobin (MYO)48T/96TY09613Chicken (Gallus)
干擾素β(IFNβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Beta (IFNb)48T/96TY09614Homo sapiens (Human)
干擾素β(IFNβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Beta (IFNb)48T/96TY09615Sus scrofa; Porcine (Pig)
足盂蛋白(PCX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Podocalyxin (PCX)48T/96TY09616Homo sapiens (Human)
前列腺抑制肽(PIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostatic Inhibin Peptide (PIP)48T/96TY09617Homo sapiens (Human)
肝素酶(HPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heparanase (HPA)48T/96TY09618Homo sapiens (Human)
絲氨酸蛋白酶1(PRSS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protease, Serine 1 (PRSS1)48T/96TY09619Mus musculus (Mouse)
血管內(nèi)皮細胞蛋白C受體(PROCR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein C Receptor, Endothelial (PROCR)48T/96TY09620Homo sapiens (Human)
蛋白C(PROC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein C (PROC)48T/96TY09621Homo sapiens (Human)
谷氨酸豐富蛋白(GRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gla Rich Protein (GRP)48T/96TY09622Homo sapiens (Human)
蛋白Z(PROZ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Z (PROZ)48T/96TY09623Homo sapiens (Human)
甘丙肽(GAL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Galanin (GAL)48T/96TY09624Rattus norvegicus (Rat)
激活蛋白C(APC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activated Protein C (APC)48T/96TY09625Homo sapiens (Human)
吲哚乙酸(IAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Indole 3 Acetic Acid (IAA)48T/96TY09626General
赤霉素(GA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gibberellic Acid (GA)48T/96TY09627General
前列腺素I合酶(PTGIS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin I Synthase (PTGIS)48T/96TY09628Homo sapiens (Human)
毒蕈堿型膽堿受體M1(CHRM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Muscarinic 1 (CHRM1)48T/96TY09629Rattus norvegicus (Rat)
煙堿型膽堿受體α1(CHRNα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1)48T/96TY09630Rattus norvegicus (Rat)
10kDa熱休克蛋白1(HSP10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 10kDa Protein 1 (HSP10)48T/96TY09631Homo sapiens (Human)
肝膠原凝集素1(CLL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Collectin Liver 1 (CLL1)48T/96TY09632Homo sapiens (Human)
肌營養(yǎng)不良蛋白(DMD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dystrophin (DMD)48T/96TY09633Homo sapiens (Human)
親環(huán)素A(CYPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclophilin A (CYPA)48T/96TY09634Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。