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人抗乙型肝炎病毒e抗體酶免試劑盒,(HBeAb)ELISA檢測試劑盒 ,英文名:HBeAb ELISA Kit ,英文縮寫:HBeAb ,常見試劑盒規(guī)格:96T/48T,產品別名:人抗乙型肝炎病毒e抗體ELISA檢測試劑盒,HBeAb 檢測試劑盒, HBeAb 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。人抗乙型肝炎病毒e抗體酶免試劑盒,(HBeAb)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重
胸腺基質淋巴細胞生成素(TSLP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thymic Stromal Lymphopoietin (TSLP)48T/96TY09235Homo sapiens (Human)
蛋白酪氨酸磷酸酶受體O(PTPRO)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase Receptor Type O (PTPRO)48T/96TY09236Homo sapiens (Human)
蛋白酪氨酸激酶2β(PTK2β)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protein Tyrosine Kinase 2 Beta (PTK2b)48T/96TY09237Homo sapiens (Human)
腎上腺素能受體α1A(ADRα1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Adrenergic Receptor Alpha 1A (ADRa1A)48T/96TY09238Rattus norvegicus (Rat)
腎上腺素能受體α1A(ADRα1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Adrenergic Receptor Alpha 1A (ADRa1A)48T/96TY09239Mus musculus (Mouse)
多巴胺受體D1(DRD1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dopamine Receptor D1 (DRD1)48T/96TY09240Rattus norvegicus (Rat)
多巴胺受體D1(DRD1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dopamine Receptor D1 (DRD1)48T/96TY09241Mus musculus (Mouse)
Ⅱ型膠原交聯羧基端肽(CTXⅡ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cross Linked C-opeptide Of Type II Collagen (CTXII)48T/96TY09242Homo sapiens (Human)
鈣調磷酸酶(CaN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Calcineurin (CaN)48T/96TY09243Homo sapiens (Human)
鈣調結合蛋白(CALD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Caldesmon (CALD)48T/96TY09244Homo sapiens (Human)
神經絲蛋白3(NEF3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neurofilament 3 (NEF3)48T/96TY09245Homo sapiens (Human)
生長關聯蛋白43(GAP43)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Associated Protein 43 (GAP43)48T/96TY09246Homo sapiens (Human)
肌腱蛋白R(TNR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tenascin R (TNR)48T/96TY09247Homo sapiens (Human)
微管關聯蛋白2(MAP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Microtubule Associated Protein 2 (MAP2)48T/96TY09248Homo sapiens (Human)
黑色素瘤關聯ME20(ME20M)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Melanoma Associated ME20 (ME20M)48T/96TY09249Homo sapiens (Human)
套膜蛋白(iNV)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Involucrin (iNV)48T/96TY09250Homo sapiens (Human)
鳥氨酸脫羧酶(ODC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ornithine Decarboxylase (ODC)48T/96TY09251Homo sapiens (Human)
粘著斑激酶(FAK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Focal Adhesion Kinase (FAK)48T/96TY09252Homo sapiens (Human)
微管關聯蛋白6(MAP6)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Microtubule Associated Protein 6 (MAP6)48T/96TY09253Homo sapiens (Human)
彈性蛋白(ELN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Elastin (ELN)48T/96TY09254Homo sapiens (Human)
晚期糖基化終末產物(AGE)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Advanced Glycation End Product (AGE)48T/96TY09255General
芳香烴受體(AhR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aryl Hydrocarbon Receptor (AhR)48T/96TY09256Homo sapiens (Human)
花生四烯酸-5-脂加氧酶(ALOX5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Arachidonate-5-Lipoxygenase (ALOX5)48T/96TY09257Homo sapiens (Human)
花生四烯酸-15-脂加氧酶B(ALOX15B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Arachidonate-15-Lipoxygenase, Type B (ALOX15B)48T/96TY09258Homo sapiens (Human)
細胞外信號調節(jié)激酶1(ERK1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Extracellular Signal Regulated Kinase 1 (ERK1)48T/96TY09259Homo sapiens (Human)
凋亡信號調節(jié)激酶I(ASK1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apoptosis Signal Regulating Kinase 1 (ASK1)48T/96TY09260Homo sapiens (Human)
酸性神經鞘磷脂酶(ASM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Acid Sphingomyelinase (ASM)48T/96TY09261Homo sapiens (Human)
毛細血管擴張性共濟失調突變因子(ATM)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ataxia angiectasia Mutated (ATM)48T/96TY09262Homo sapiens (Human)
精氨酸加壓素受體1A(AVPR1A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Arginine Vasopressin Receptor 1A (AVPR1A)48T/96TY09263Homo sapiens (Human)
原鈣黏素1(PCDH1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protocadherin 1 (PCDH1)48T/96TY09264Homo sapiens (Human)
乳腺癌易感蛋白2(BRCA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Breast Cancer Susceptibility Protein 2 (BRCA2)48T/96TY09265Homo sapiens (Human)
葡萄糖激酶調節(jié)蛋白(GKRP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glucokinase Regulatory Protein (GKRP)48T/96TY09266Homo sapiens (Human)
Bcl2關聯X蛋白(Bax)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Bcl2 Associated X Protein (Bax)48T/96TY09267Sus scrofa; Porcine (Pig)
B-細胞淋巴瘤因子2(Bcl2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2)48T/96TY09268Sus scrofa; Porcine (Pig)
凋亡相關因子(FAS)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Factor Related Apoptosis (FAS)48T/96TY09269Sus scrofa; Porcine (Pig)
血管內皮鈣黏蛋白(CDH5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cadherin 5 (CDH5)48T/96TY09270Homo sapiens (Human)
血管內皮鈣黏蛋白(CDH5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cadherin 5 (CDH5)48T/96TY09271Sus scrofa; Porcine (Pig)
血管內皮生長因子受體2(VEGFR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)48T/96TY09272Homo sapiens (Human)
血管內皮生長因子受體2(VEGFR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)48T/96TY09273Sus scrofa; Porcine (Pig)
肝配蛋白A受體1(EPHA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ephrin Type A Receptor 1 (EPHA1)48T/96TY09274Homo sapiens (Human)
肝配蛋白A受體1(EPHA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ephrin Type A Receptor 1 (EPHA1)48T/96TY09275Sus scrofa; Porcine (Pig)
乳酸脫氫酶A(LDHA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lactate Dehydrogenase A (LDHA)48T/96TY09276Homo sapiens (Human)
轉醛縮酶(TALDO1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transaldolase (TALDO1)48T/96TY09277Homo sapiens (Human)
鱗狀細胞癌抗原1(SCCA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Squamous Cell Carcinoma Antigen 1 (SCCA1)48T/96TY09278Homo sapiens (Human)
防御素β1(DEFβ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Defensin Beta 1 (DEFb1)48T/96TY09279Rattus norvegicus (Rat)
小核糖核蛋白多肽C(SNRPC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Small Nuclear Ribonucleoprotein Polypeptide C (SNRPC)48T/96TY09280Homo sapiens (Human)
γ-谷氨酰轉移酶1(γGT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gamma-Glutamyltransferase 1 (gGT1)48T/96TY09281Homo sapiens (Human)
乳腺癌易感蛋白1(BRCA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Breast Cancer Susceptibility Protein 1 (BRCA1)48T/96TY09282Homo sapiens (Human)
間羥腎上腺素(MN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Metanephrine (MN)48T/96TY09283General
垂草扁桃酸(VMA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vanillylmandelic Acid (VMA)48T/96TY09284General各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。